More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Hec1 antibody

Hec1 antibody

GTX110735
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNDC80
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Hec1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.46 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10403
  • Target name
    NDC80
  • Target description
    NDC80 kinetochore complex component
  • Target synonyms
    HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80, kinetochore protein NDC80 homolog, NDC80 homolog, kinetochore complex component, NDC80 kinetochore complex component homolog, highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, kinetochore associated 2, kinetochore protein Hec1, kinetochore-associated protein 2, retinoblastoma-associated protein HEC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14777
  • Protein Name
    Kinetochore protein NDC80 homolog
  • Scientific Description
    HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Liu YH, Weng YP, Tsai HY, et al. Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells. Phytomedicine. 2018,46:184-192. doi: 10.1016/j.phymed.2018.03.037
    Read this paper
  • Lee YS, Chuang SH, Huang LY, et al. Discovery of 4-aryl-N-arylcarbonyl-2-aminothiazoles as Hec1/Nek2 inhibitors. Part I: optimization of in vitro potencies and pharmacokinetic properties. J Med Chem. 2014,57(10):4098-110. doi: 10.1021/jm401990s
    Read this paper
  • Huang LY, Lee YS, Huang JJ, et al. Characterization of the biological activity of a potent small molecule Hec1 inhibitor TAI-1. J Exp Clin Cancer Res. 2014,33(1):6. doi: 10.1186/1756-9966-33-6
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Bioss
HEC1 Recombinant Antibody, AbBy Fluor-488 ConjugatedBSM-61551R-BF488
ApplicationsWestern Blot
TargetNDC80
  • SizePrice
Product group Antibodies
Assay Genie
HEC1/NDC80 Monoclonal AntibodyCAB2392
TargetNDC80
  • SizePrice
Figure 1. Western blot analysis of NDC80 using anti-NDC80 antibody (A01731-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDC80 antigen affinity purified polyclonal antibody (Catalog # A01731-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDC80 at approximately 74KD. The expected band size for NDC80 is at 74KD.
Product group Antibodies
Boster Bio
Anti-HEC1/HEC/NDC80 Antibody Picoband(r)A01731-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetNDC80
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NDC80 Antibody144-05411
ApplicationsWestern Blot
TargetNDC80
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Kinetochore protein NDC80 homolog [Hec1 (9G3)]AB03500-1.1-BT
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
TargetNDC80
  • SizePrice
Anti-KNTC2 Antibody
Product group Antibodies
Antibodies.com
Anti-KNTC2 AntibodyA97839
ApplicationsImmunoFluorescence, Western Blot, ELISA
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-HEC1EB05610
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetNDC80
  • SizePrice
Product group Antibodies
GeneTex
Hec1 (non-phospho Ser 165) antibodyGTX70012
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetNDC80
  • SizePrice
Product group Antibodies
References
GeneTex
Hec1 (phospho Ser 165) antibodyGTX70013
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetNDC80
  • SizePrice
Product group Antibodies
GeneTex
Hec1 (non-phospho Ser 76/77) antibodyGTX70014
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetNDC80
  • SizePrice