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Goat anti-HEC1

EB05610
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNDC80
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-HEC1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10403
  • Target name
    NDC80
  • Target description
    NDC80 kinetochore complex component
  • Target synonyms
    HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80, kinetochore protein NDC80 homolog, NDC80 homolog, kinetochore complex component, NDC80 kinetochore complex component homolog, highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, kinetochore associated 2, kinetochore protein Hec1, kinetochore-associated protein 2, retinoblastoma-associated protein HEC
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006092.1. Purification: Antigen affinity purified. Names and symbols: NDC80; HEC1; HEC; highly expressed in cancer, rich in leucine heptad repeats; HEC-1; KNTC2; NDC80 homolog, kinetochore complex component (S. cerevisiae); TID3; hsNDC80; kinetochore associated 2
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDC80 using anti-NDC80 antibody (A01731-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDC80 antigen affinity purified polyclonal antibody (Catalog # A01731-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDC80 at approximately 74KD. The expected band size for NDC80 is at 74KD.
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