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Anti-Lamin B1 Antibody

R1508-1
HUABIO
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetLMNB1
100 ul
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Overview

  • Supplier
    HUABIO
  • Product Name
    Anti-Lamin B1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4001
  • Target name
    LMNB1
  • Target description
    lamin B1
  • Target synonyms
    ADLD, ADLDAT, LMN, LMN2, LMNB, MCPH26, lamin-B1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20700
  • Protein Name
    Lamin-B1
  • Scientific Description
    Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Lamin B, along with heterochromatin, is anchored to the inner surface of the nuclear membrane by the lamin B receptor. LMNB1 has been shown to interact with Thymopoietin.
  • Reactivity
    Human, Rat
  • Reactivity Supplier
    Human,Rat
  • Reactivity Supplier Note
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody (Catalog # PB9611) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B1 at approximately 72 kDa. The expected band size for Lamin B1 is at 66 kDa.
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