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Anti-Laminin [A5]

AB02770-7.1-BT
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Mouse
TargetLAMC1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Laminin [A5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This original rat IgG2a clone can be used to detect and analyze the presence of laminin. The specificity towards this protein has been confirmed using immunoprecipitation (Ljubimov et al., 1986; PMID: 3522258). The location of the laminin was confirmed to be in the basment membrane by means of immunofluorescence using this antibody (Eriksdotter-Nielson et al., 1986; PMID: 3537540).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A5
  • Gene ID3915
  • Target name
    LAMC1
  • Target description
    laminin subunit gamma 1
  • Target synonyms
    LAMB2, laminin subunit gamma-1, S-LAM gamma, S-laminin subunit gamma, laminin B2 chain, laminin, gamma 1 (formerly LAMB2), laminin-10 subunit gamma, laminin-11 subunit gamma, laminin-2 subunit gamma, laminin-3 subunit gamma, laminin-4 subunit gamma, laminin-6 subunit gamma, laminin-7 subunit gamma, laminin-8 subunit gamma, laminin-9 subunit gamma
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP11047
  • Protein Name
    Laminin subunit gamma-1
  • Reactivity
    Bovine, Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Laminin Gamma 1/LAMC1 using anti-Laminin Gamma 1/LAMC1 antibody (A03522-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin Gamma 1/LAMC1 antigen affinity purified polyclonal antibody (Catalog # A03522-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Laminin Gamma 1/LAMC1 at approximately 200 kDa. The expected band size for Laminin Gamma 1/LAMC1 is at 178 kDa.
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