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Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.

Anti-LGR5 Antibody

HPA012530
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLGR5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LGR5
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8549
  • Target name
    LGR5
  • Target description
    leucine rich repeat containing G protein-coupled receptor 5
  • Target synonyms
    FEX, GPR49, GPR67, GRP49, HG38, leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, orphan G protein-coupled receptor HG38
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75473
  • Protein Name
    Leucine-rich repeat-containing G-protein coupled receptor 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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IHC image of CSB-PA012906LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of LGR5 using anti-LGR5 antibody (A00239-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LGR5 antigen affinity purified polyclonal antibody (Catalog # A00239-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LGR5 at approximately 130 kDa. The expected band size for LGR5 is at 100,97,92 kDa.
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