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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.

Anti-LOXL2 Antibody

HPA056542
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetLOXL2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LOXL2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4017
  • Target name
    LOXL2
  • Target description
    lysyl oxidase like 2
  • Target synonyms
    LOR, LOR2, WS9-14, lysyl oxidase homolog 2, lysyl oxidase related 2, lysyl oxidase-like 2 delta e13, lysyl oxidase-like 2 protein, lysyl oxidase-like protein 2, lysyl oxidase-related protein 2, lysyl oxidase-related protein WS9-14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4K0
  • Protein Name
    Lysyl oxidase homolog 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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LOXL2 antibody detects LOXL2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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Figure 1. Western blot analysis of LOXL2 using anti-LOXL2 antibody (PB9720). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOXL2 antigen affinity purified polyclonal antibody (Catalog # PB9720) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOXL2 at approximately 105 kDa. The expected band size for LOXL2 is at 87 kDa.
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