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Immunofluorescent staining of human cell line ASC TERT1 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line ASC TERT1 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line ASC TERT1 shows localization to nucleoplasm.

Anti-LRRC32 Antibody

HPA055775
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLRRC32
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LRRC32
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2615
  • Target name
    LRRC32
  • Target description
    leucine rich repeat containing 32
  • Target synonyms
    CPPRDD; D11S833E; GARP; garpin; glycoprotein A repetitions predominant; leucine-rich repeat-containing protein 32; transforming growth factor beta activator LRRC32
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14392
  • Protein Name
    Leucine-rich repeat-containing protein 32
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRRC32 using anti-LRRC32 antibody (A08199-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC32 antigen affinity purified polyclonal antibody (Catalog # A08199-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC32 at approximately 72KD. The expected band size for LRRC32 is at 72KD.
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