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ProductsBack/LRRC32 Antibody (HRP)

LRRC32 Antibody (HRP)

LS-C395166
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetLRRC32
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    LRRC32 Antibody (HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID2615
  • Target name
    LRRC32
  • Target description
    leucine rich repeat containing 32
  • Target synonyms
    CPPRDD, D11S833E, GARP, transforming growth factor beta activator LRRC32, garpin, glycoprotein A repetitions predominant, leucine-rich repeat-containing protein 32
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRRC32 using anti-LRRC32 antibody (A08199-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC32 antigen affinity purified polyclonal antibody (Catalog # A08199-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC32 at approximately 72KD. The expected band size for LRRC32 is at 72KD.
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