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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA618886LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA618886LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA618886LA01HU at dilution of 1:100

LRRC32 Antibody

CSB-PA618886LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLRRC32
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Overview

  • Supplier
    Cusabio
  • Product Name
    LRRC32 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2615
  • Target name
    LRRC32
  • Target description
    leucine rich repeat containing 32
  • Target synonyms
    CPPRDD; D11S833E; GARP; garpin; glycoprotein A repetitions predominant; leucine-rich repeat-containing protein 32; transforming growth factor beta activator LRRC32
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14392
  • Protein Name
    Leucine-rich repeat-containing protein 32
  • Scientific Description
    Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space (PubMed:19750484, PubMed:19651619, PubMed:22278742). Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta (PubMed:22278742). Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta (PubMed:22278742). Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs) (PubMed:19750484, PubMed:19651619). Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRRC32 using anti-LRRC32 antibody (A08199-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC32 antigen affinity purified polyclonal antibody (Catalog # A08199-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC32 at approximately 72KD. The expected band size for LRRC32 is at 72KD.
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