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Immunofluorescent staining of human cell line RT4 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line RT4 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line RT4 shows localization to the Golgi apparatus.

Anti-LTBR Antibody

HPA061617
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetLTBR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LTBR Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4055
  • Target name
    LTBR
  • Target description
    lymphotoxin beta receptor
  • Target synonyms
    D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFR3, TNFRSF3, tumor necrosis factor receptor superfamily member 3, lymphotoxin B receptor, lymphotoxin beta receptor (TNFR superfamily, member 3), tumor necrosis factor C receptor, tumor necrosis factor receptor 2-related protein, tumor necrosis factor receptor type III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36941
  • Protein Name
    Tumor necrosis factor receptor superfamily member 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of LTBR using anti-LTBR antibody (A00942-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hut-78 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTBR antigen affinity purified polyclonal antibody (Catalog # A00942-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTBR at approximately 55-60 kDa. The expected band size for LTBR is at 47 kDa.
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