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Western Blot analysis of HepG2 cells using LTbetaR Polyclonal Antibody
Western Blot analysis of HepG2 cells using LTbetaR Polyclonal Antibody
Western Blot analysis of HepG2 cells using LTbetaR Polyclonal Antibody

LTBR Antibody

CSB-PA003174
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetLTBR
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Overview

  • Supplier
    Cusabio
  • Product Name
    LTBR Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4055
  • Target name
    LTBR
  • Target description
    lymphotoxin beta receptor
  • Target synonyms
    D12S370; LT-BETA-R; lymphotoxin B receptor; lymphotoxin beta receptor (TNFR superfamily, member 3); TNFCR; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; tumor necrosis factor C receptor; tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor type III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36941
  • Protein Name
    Tumor necrosis factor receptor superfamily member 3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LTBR using anti-LTBR antibody (A00942-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hut-78 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTBR antigen affinity purified polyclonal antibody (Catalog # A00942-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTBR at approximately 55-60 kDa. The expected band size for LTBR is at 47 kDa.
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Lane 1: HepG2, Lane 2: Mouse cerebrum lysates probed with LTBR Polyclonal Antibody (bs-6917R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
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