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LTBR Antibody (YA3985, Mouse)

HY-P84288
MedChem Express
TargetLTBR
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    LTBR Antibody (YA3985, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4055
  • Target name
    LTBR
  • Target description
    lymphotoxin beta receptor
  • Target synonyms
    D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFR3, TNFRSF3, tumor necrosis factor receptor superfamily member 3, lymphotoxin B receptor, lymphotoxin beta receptor (TNFR superfamily, member 3), tumor necrosis factor C receptor, tumor necrosis factor receptor 2-related protein, tumor necrosis factor receptor type III
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    LTBR Antibody (YA3985) is a mouse-derived and non-conjugated IgG2a antibody, targeting to LTBR. It can be applicated for WB, FC, ELISA assays, in the background of human, mouse.
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of LTBR using anti-LTBR antibody (A00942-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hut-78 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTBR antigen affinity purified polyclonal antibody (Catalog # A00942-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTBR at approximately 55-60 kDa. The expected band size for LTBR is at 47 kDa.
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