Figure 2. Flow Cytometry analysis of PC-3 cells using anti-MAG antibody (A03019). Overlay histogram showing PC-3 cells stained with A03019 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAG Antibody (A03019, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 2. Flow Cytometry analysis of PC-3 cells using anti-MAG antibody (A03019). Overlay histogram showing PC-3 cells stained with A03019 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAG Antibody (A03019, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Anti-MAG Antibody Picoband(r)
A03019-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetMAG
Overview
- SupplierBoster Bio
- Product NameAnti-MAG Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID4099
- Target nameMAG
- Target descriptionmyelin associated glycoprotein
- Target synonymsGMA, S-MAG, SIGLEC-4A, SIGLEC4, SIGLEC4A, SPG75, myelin-associated glycoprotein, sialic acid binding Ig-like lectin 4A, sialic acid-binding immunoglobulin-like lectin 4A
- HostRabbit
- IsotypeIgG
- Protein IDP20916
- Protein NameMyelin-associated glycoprotein
- Scientific DescriptionBoster Bio Anti-MAG Antibody Picoband® catalog # A03019. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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