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Western Blot analysis of HeLa HepG2 cells using MAG Polyclonal Antibody
Western Blot analysis of HeLa HepG2 cells using MAG Polyclonal Antibody
Western Blot analysis of HeLa HepG2 cells using MAG Polyclonal Antibody

MAG Antibody

CSB-PA006022
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAG
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAG Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4099
  • Target name
    MAG
  • Target description
    myelin associated glycoprotein
  • Target synonyms
    GMA, S-MAG, SIGLEC-4A, SIGLEC4, SIGLEC4A, SPG75, myelin-associated glycoprotein, sialic acid binding Ig-like lectin 4A, sialic acid-binding immunoglobulin-like lectin 4A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20916
  • Protein Name
    Myelin-associated glycoprotein
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-MAG antibody (A03019). Overlay histogram showing PC-3 cells stained with A03019 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAG Antibody (A03019, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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