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SH-SY5Y cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MAG Polyclonal Antibody(bs-0257R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
SH-SY5Y cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MAG Polyclonal Antibody(bs-0257R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
SH-SY5Y cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MAG Polyclonal Antibody(bs-0257R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

MAG Polyclonal Antibody

BS-0257R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMAG
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MAG Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4099
  • Target name
    MAG
  • Target description
    myelin associated glycoprotein
  • Target synonyms
    GMA; myelin-associated glycoprotein; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A; SIGLEC4A; SIGLEC-4A; S-MAG; SPG75
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20916
  • Protein Name
    Myelin-associated glycoprotein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • MicroRNA-210 contributes to peripheral nerve regeneration through promoting the proliferation and migration of Schwann cells. Zhang X et al., 2017 Oct, Exp Ther Med
    Read more
  • Capparis ovata treatment suppresses inflammatory cytokine expression and ameliorates experimental allergic encephalomyelitis model of multiple sclerosis in C57BL/6 mice. Ozgun-Acar O et al., 2016 Sep 15, J Neuroimmunol
    Read more
  • Transplantation of mesenchymal stem cells promotes the functional recovery of the central nervous system following cerebral ischemia by inhibiting myelin-associated inhibitor expression and neural apoptosis. Feng N et al., 2016 May, Exp Ther Med
    Read more

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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-MAG antibody (A03019). Overlay histogram showing PC-3 cells stained with A03019 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAG Antibody (A03019, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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