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MAG antibody detects MAG protein at on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. MAG antibody (GTX114542) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
MAG antibody detects MAG protein at on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. MAG antibody (GTX114542) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
MAG antibody detects MAG protein at on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. MAG antibody (GTX114542) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

MAG antibody

GTX114542
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAG
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Overview

  • Supplier
    GeneTex
  • Product Name
    MAG antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.61 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4099
  • Target name
    MAG
  • Target description
    myelin associated glycoprotein
  • Target synonyms
    GMA, S-MAG, SIGLEC-4A, SIGLEC4, SIGLEC4A, SPG75, myelin-associated glycoprotein, sialic acid binding Ig-like lectin 4A, sialic acid-binding immunoglobulin-like lectin 4A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20916
  • Protein Name
    Myelin-associated glycoprotein
  • Scientific Description
    The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-MAG antibody (A03019). Overlay histogram showing PC-3 cells stained with A03019 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAG Antibody (A03019, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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