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Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.

Anti-MAPK7 Antibody

HPA031031
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMAPK7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MAPK7
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5598
  • Target name
    MAPK7
  • Target description
    mitogen-activated protein kinase 7
  • Target synonyms
    big MAP kinase 1; BMK1; BMK-1; BMK1 kinase; ERK4; ERK5; ERK-5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK 7; mitogen-activated protein kinase 7; PRKM7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13164
  • Protein Name
    Mitogen-activated protein kinase 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERK5/MAPK7 using anti-ERK5/MAPK7 antibody (A02812-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hek293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey Cos-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERK5/MAPK7 antigen affinity purified polyclonal antibody (Catalog # A02812-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERK5/MAPK7 at approximately 115-120KD. The expected band size for ERK5/MAPK7 is at 88KD.
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Lane 1: Human HepG2 cell lysates; Lane 2: Human Hcclm3 cell lysates probed with ERK5 (Ser496) Polyclonal Antibody, Unconjugated (bs-5484R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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