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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA013465ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA013465ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA013465ESR1HU at dilution of 1:100

MAPK7 Antibody

CSB-PA013465ESR1HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMAPK7
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAPK7 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5598
  • Target name
    MAPK7
  • Target description
    mitogen-activated protein kinase 7
  • Target synonyms
    big MAP kinase 1; BMK1; BMK-1; BMK1 kinase; ERK4; ERK5; ERK-5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK 7; mitogen-activated protein kinase 7; PRKM7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13164
  • Protein Name
    Mitogen-activated protein kinase 7
  • Scientific Description
    Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERK5/MAPK7 using anti-ERK5/MAPK7 antibody (A02812-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hek293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey Cos-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERK5/MAPK7 antigen affinity purified polyclonal antibody (Catalog # A02812-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERK5/MAPK7 at approximately 115-120KD. The expected band size for ERK5/MAPK7 is at 88KD.
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Lane 1: Human HepG2 cell lysates; Lane 2: Human Hcclm3 cell lysates probed with ERK5 (Ser496) Polyclonal Antibody, Unconjugated (bs-5484R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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