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ProductsBack/phospho-ERK5 (Ser496) Polyclonal Antibody
Lane 1: Human HepG2 cell lysates; Lane 2: Human Hcclm3 cell lysates probed with ERK5 (Ser496) Polyclonal Antibody, Unconjugated (bs-5484R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human Hcclm3 cell lysates probed with ERK5 (Ser496) Polyclonal Antibody, Unconjugated (bs-5484R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human Hcclm3 cell lysates probed with ERK5 (Ser496) Polyclonal Antibody, Unconjugated (bs-5484R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

phospho-ERK5 (Ser496) Polyclonal Antibody

BS-5484R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAPK7
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Overview

  • Supplier
    Bioss
  • Product Name
    phospho-ERK5 (Ser496) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5598
  • Target name
    MAPK7
  • Target description
    mitogen-activated protein kinase 7
  • Target synonyms
    BMK1, ERK4, ERK5, PRKM7, mitogen-activated protein kinase 7, BMK-1, BMK1 kinase, ERK-5, MAP kinase 7, MAPK 7, big MAP kinase 1, extracellular-signal-regulated kinase 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13164
  • Protein Name
    Mitogen-activated protein kinase 7
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhuang K, Zhang J, Xiong M, et al. CDK5 functions as a tumor promoter in human colorectal cancer via modulating the ERK5-AP-1 axis. Cell Death Dis. 2016,7(10):e2415. doi: 10.1038/cddis.2016.333
    Read this paper

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Figure 1. Western blot analysis of ERK5/MAPK7 using anti-ERK5/MAPK7 antibody (A02812-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hek293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey Cos-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERK5/MAPK7 antigen affinity purified polyclonal antibody (Catalog # A02812-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERK5/MAPK7 at approximately 115-120KD. The expected band size for ERK5/MAPK7 is at 88KD.
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