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Western blot analysis of lysates from Jurkat cells, using MKP-1/2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using MKP-1/2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using MKP-1/2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-MKP-1/2 DUSP1 Antibody

A30451
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDUSP1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-MKP-1/2 DUSP1 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Gene ID1843
  • Target name
    DUSP1
  • Target description
    dual specificity phosphatase 1
  • Target synonyms
    CL100, HVH1, MKP-1, MKP1, PTPN10, dual specificity protein phosphatase 1, CL 100, MAP kinase phosphatase 1, dual specificity protein phosphatase hVH1, mitogen-activated protein kinase phosphatase 1, protein-tyrosine phosphatase CL100, serine/threonine specific protein phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28562
  • Protein Name
    Dual specificity protein phosphatase 1
  • Scientific Description
    Boster Bio Anti-MKP-1/2 DUSP1 Antibody (Catalog# A30451). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Lin S, Chen Z, Wu Z, et al. Involvement of PI3K/AKT Pathway in the Rapid Antidepressant Effects of Crocetin in Mice with Depression-Like Phenotypes. Neurochem Res. 2024,49(2):477-491. doi: 10.1007/s11064-023-04051-2
    Read this paper
  • Zhang Q, Abouelfetouh MM, Chen S, et al. MicroRNA Let-7b-5p Induces Electroacupuncture Tolerance by Downregulating the MKP-1 Gene in Rats Subjected to CFA-induced Inflammatory Nociception. J Mol Neurosci. 2020,70(8):1198-1207. doi: 10.1007/s12031-020-01527-6
    Read this paper

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Figure 1. Western blot analysis of MKP-1/DUSP1 using anti-MKP-1/DUSP1 antibody (A02276-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKP-1/DUSP1 antigen affinity purified polyclonal antibody (Catalog # A02276-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKP-1/DUSP1 at approximately 40KD. The expected band size for MKP-1/DUSP1 is at 40KD.
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