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Immunohistochemical staining of human heart muscle shows positivity in cardiomyocytes.
Immunohistochemical staining of human heart muscle shows positivity in cardiomyocytes.
Immunohistochemical staining of human heart muscle shows positivity in cardiomyocytes.

Anti-MT-CO1 Antibody

HPA069328
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCOX1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MT-CO1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4512
  • Target name
    COX1
  • Target description
    cytochrome c oxidase subunit I
  • Target synonyms
    COI; cytochrome c oxidase subunit I; MTCO1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00395
  • Protein Name
    Cytochrome c oxidase subunit 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MTCO1 using anti-MTCO1 antibody (PA1317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The samples were loaded under reducing conditions. Lane 1: human HeLa mitochondria lysates at 20ug, Lane 2: human HeLa whole cell lysates at 20ug, Lane 3: human Caco-2 whole cell lysates at 50ug, Lane 4: rat heart tissue lysates at 50ug. Lane 5: mouse heart tissue lysates at 50ug. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 60 minutes. Blocked the membrane with 5% Non-fat Milk in TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTCO1 antigen affinity purified polyclonal antibody (Catalog # PA1317-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTCO1 at approximately 37KD. The expected band size for MTCO1 is at 57KD.
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