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MT-CO1 / COX1 Antibody

LS-C668936
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCOX1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MT-CO1 / COX1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of COX1 expression in Hela (A), mouse brain (B) whole cell lysates.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Western blot analysis of COX1 expression in Hela (A), mouse brain (B) whole cell lysates.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4512
  • Target name
    COX1
  • Target description
    cytochrome c oxidase subunit I
  • Target synonyms
    COI, MTCO1, cytochrome c oxidase subunit I
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MTCO1 using anti-MTCO1 antibody (PA1317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The samples were loaded under reducing conditions. Lane 1: human HeLa mitochondria lysates at 20ug, Lane 2: human HeLa whole cell lysates at 20ug, Lane 3: human Caco-2 whole cell lysates at 50ug, Lane 4: rat heart tissue lysates at 50ug. Lane 5: mouse heart tissue lysates at 50ug. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 60 minutes. Blocked the membrane with 5% Non-fat Milk in TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTCO1 antigen affinity purified polyclonal antibody (Catalog # PA1317-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTCO1 at approximately 37KD. The expected band size for MTCO1 is at 57KD.
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