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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA015072LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA015072LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA015072LA01HU at dilution of 1:100

MT-CO1 Antibody

CSB-PA015072LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCOX1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MT-CO1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4512
  • Target name
    COX1
  • Target description
    cytochrome c oxidase subunit I
  • Target synonyms
    COI, MTCO1, cytochrome c oxidase subunit I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00395
  • Protein Name
    Cytochrome c oxidase subunit 1
  • Scientific Description
    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MTCO1 using anti-MTCO1 antibody (PA1317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The samples were loaded under reducing conditions. Lane 1: human HeLa mitochondria lysates at 20ug, Lane 2: human HeLa whole cell lysates at 20ug, Lane 3: human Caco-2 whole cell lysates at 50ug, Lane 4: rat heart tissue lysates at 50ug. Lane 5: mouse heart tissue lysates at 50ug. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 60 minutes. Blocked the membrane with 5% Non-fat Milk in TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTCO1 antigen affinity purified polyclonal antibody (Catalog # PA1317-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTCO1 at approximately 37KD. The expected band size for MTCO1 is at 57KD.
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WB analysis of various samples using GTX11619 MTCO1 antibody. Lane 1 : MCF-7 cell lysate Lane 2 : HeLa cell lysate Lane 3 : Jurkat cell lysate Lane 4 : SMMC cell lysate Lane 5 : COLO320 cell lysate
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Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX1/MTCO1 Polyclonal Antibody, Unconjugated (bs-3953R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
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Immunohistochemical staining of human heart muscle shows positivity in cardiomyocytes.
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