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Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.

Anti-MYB Antibody

HPA071605
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMYB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MYB
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4602
  • Target name
    MYB
  • Target description
    MYB proto-oncogene, transcription factor
  • Target synonyms
    Cmyb, c-myb, c-myb_CDS, efg, transcriptional activator Myb, MYB-GATA1 fusion protein, oncogene AMV, proto-oncogene c-Myb, v-myb avian myeloblastosis viral oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10242
  • Protein Name
    Transcriptional activator Myb
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of c-Myb using anti-c-Myb antibody (PB9288). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Myb antigen affinity purified polyclonal antibody (Catalog # PB9288) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Myb at approximately 74 kDa. The expected band size for c-Myb is at 74 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with B MyB Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, DAB staining.
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