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Anti-c-Myb Antibody

188-10912
RayBiotech
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-c-Myb Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Certification
    Research Use Only
  • Concentration
    0.2 mg/ml
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP01104
  • Protein Name
    Transforming protein Myb
  • Scientific Description
    Mouse anti-Human c-Myb Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of c-Myb using anti-c-Myb antibody (PB9288). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Myb antigen affinity purified polyclonal antibody (Catalog # PB9288) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Myb at approximately 74 kDa. The expected band size for c-Myb is at 74 kDa.
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