Bio-Connect

MYB / c-Myb Antibody

LS-C748801
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMYB
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MYB / c-Myb Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 37-46kDa/63-85kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 37-46kDa/63-85kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4602
  • Target name
    MYB
  • Target description
    MYB proto-oncogene, transcription factor
  • Target synonyms
    Cmyb; c-myb; c-myb_CDS; efg; oncogene AMV; proto-oncogene c-Myb; transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Anti-c-Myb Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetMYB
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, ImmunoHistoChemistry
TargetMYB
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with B MyB Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, DAB staining.
Product group Antibodies
References
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMYB
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMYB
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetMYB
  • SizePrice
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry
TargetMYB
  • SizePrice
Figure 1. Western blot analysis of c-Myb using anti-c-Myb antibody (PB9288). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Myb antigen affinity purified polyclonal antibody (Catalog # PB9288) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Myb at approximately 74 kDa. The expected band size for c-Myb is at 74 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetMYB
  • SizePrice