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Western Blot analysis of Jurkat cells using c-Myb Polyclonal Antibody
Western Blot analysis of Jurkat cells using c-Myb Polyclonal Antibody
Western Blot analysis of Jurkat cells using c-Myb Polyclonal Antibody

MYB Antibody

CSB-PA005513
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetMYB
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Overview

  • Supplier
    Cusabio
  • Product Name
    MYB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4602
  • Target name
    MYB
  • Target description
    MYB proto-oncogene, transcription factor
  • Target synonyms
    Cmyb, c-myb, c-myb_CDS, efg, transcriptional activator Myb, MYB-GATA1 fusion protein, oncogene AMV, proto-oncogene c-Myb, v-myb avian myeloblastosis viral oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10242
  • Protein Name
    Transcriptional activator Myb
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of c-Myb using anti-c-Myb antibody (PB9288). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Myb antigen affinity purified polyclonal antibody (Catalog # PB9288) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Myb at approximately 74 kDa. The expected band size for c-Myb is at 74 kDa.
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