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Anti-MZB1 Antibody

144-63304
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMZB1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MZB1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID51237
  • Target name
    MZB1
  • Target description
    marginal zone B and B1 cell specific protein
  • Target synonyms
    MEDA-7, PACAP, pERp1, marginal zone B- and B1-cell-specific protein, HSPC190, caspase-2 binding protein, mesenteric estrogen-dependent adipose 7, mesenteric oestrogen-dependent adipose gene- 7, plasma cell-induced ER protein 1, plasma cell-induced resident ER protein, plasma cell-induced resident endoplasmic reticulum protein, proapoptotic caspase adapter protein, proapoptotic caspase adaptor protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WU39
  • Protein Name
    Marginal zone B- and B1-cell-specific protein
  • Scientific Description
    MZB1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MZB1 using anti-MZB1 antibody (A08281-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MZB1 antigen affinity purified polyclonal antibody (Catalog # A08281-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MZB1 at approximately 19 kDa. The expected band size for MZB1 is at 21 kDa.
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