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Immunohistochemical staining of human Pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human Pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human Pancreas shows strong nuclear positivity in exocrine glandular cells.

Anti-NANS Antibody

HPA019223
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNANS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NANS
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54187
  • Target name
    NANS
  • Target description
    N-acetylneuraminate synthase
  • Target synonyms
    HEL-S-100, SAS, SEMDCG, SEMDG, N-acetylneuraminate-9-phosphate synthase, 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate synthase, N-acetylneuraminic acid phosphate synthase, N-acetylneuraminic acid synthase, epididymis secretory protein Li 100, sialic acid phosphate synthase, sialic acid synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR45
  • Protein Name
    Sialic acid synthase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NANS using anti-NANS antibody (A08540-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NANS antigen affinity purified polyclonal antibody (A08540-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NANS at approximately 38 kDa. The expected band size for NANS is at 40 kDa.
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