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Anti-NANS Antibody

CAB4778
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNANS
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-NANS Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID54187
  • Target name
    NANS
  • Target description
    N-acetylneuraminate synthase
  • Target synonyms
    HEL-S-100, SAS, SEMDCG, SEMDG, N-acetylneuraminate-9-phosphate synthase, 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate synthase, N-acetylneuraminic acid phosphate synthase, N-acetylneuraminic acid synthase, epididymis secretory protein Li 100, sialic acid phosphate synthase, sialic acid synthase
  • Host
    Rabbit
  • Protein IDQ9NR45
  • Protein Name
    N-acetylneuraminate-9-phosphate synthase
  • Scientific Description
    NANS Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NANS using anti-NANS antibody (A08540-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NANS antigen affinity purified polyclonal antibody (A08540-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NANS at approximately 38 kDa. The expected band size for NANS is at 40 kDa.
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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: NANS antibody at 5.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa
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