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NANS Polyclonal Antibody

CAC13524
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNANS
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Overview

  • Supplier
    Biomatik
  • Product Name
    NANS Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54187
  • Target name
    NANS
  • Target description
    N-acetylneuraminate synthase
  • Target synonyms
    HEL-S-100, SAS, SEMDCG, SEMDG, N-acetylneuraminate-9-phosphate synthase, 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate synthase, N-acetylneuraminic acid phosphate synthase, N-acetylneuraminic acid synthase, epididymis secretory protein Li 100, sialic acid phosphate synthase, sialic acid synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR45
  • Protein Name
    Sialic acid synthase
  • Scientific Description
    The NANS Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: NANS antibody at 5.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa
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Figure 1. Western blot analysis of NANS using anti-NANS antibody (A08540-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NANS antigen affinity purified polyclonal antibody (A08540-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NANS at approximately 38 kDa. The expected band size for NANS is at 40 kDa.
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