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Immunohistochemical staining of human skin shows strong membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong membranous positivity in squamous epithelial cells.

Anti-NECTIN1 Antibody

HPA026846
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNECTIN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NECTIN1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5818
  • Target name
    NECTIN1
  • Target description
    nectin cell adhesion molecule 1
  • Target synonyms
    CD111; CLPED1; ectodermal dysplasia 4 (Margarita Island type); ED4; herpes simplex virus type 1 sensitivity; herpes virus entry mediator C; herpesvirus Ig-like receptor; HIgR; HV1S; HVEC; nectin 1; nectin-1; OFC7; poliovirus receptor-like 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15223
  • Protein Name
    Nectin-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (A04988-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human Sw620 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human SGC-7901 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse spleen tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates, Lane 10: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PVRL1/NECTIN1 antigen affinity purified polyclonal antibody (Catalog # A04988-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PVRL1/NECTIN1 at approximately 110KD. The expected band size for PVRL1/NECTIN1 is at 110KD.
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