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PVRL1 antibody

10R-5535
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    PVRL1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PVRL1/NECTIN1 using anti-PVRL1/NECTIN1 antibody (A04988-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human Sw620 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human SGC-7901 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse spleen tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates, Lane 10: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PVRL1/NECTIN1 antigen affinity purified polyclonal antibody (Catalog # A04988-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PVRL1/NECTIN1 at approximately 110KD. The expected band size for PVRL1/NECTIN1 is at 110KD.
Product group Antibodies
Boster Bio
Anti-PVRL1/NECTIN1 Antibody Picoband(r)A04988-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNECTIN1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PVRL1 Antibody144-65455
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetNECTIN1
  • SizePrice
Western Blot analysis of KB cells using Nectin 1 Polyclonal Antibody
Product group Antibodies
Cusabio
PVRL1 AntibodyCSB-PA006247
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetNECTIN1
  • SizePrice
Product group Antibodies
Assay Genie
HLA-DQB1 AntibodyCAB20372
ReactivityHuman
TargetNECTIN1
  • SizePrice
Lane 1: Mouse Urinary bladder tissue lysates; Lane 2: Rat Urinary bladder tissue lysates probed with PVRL1/CD111 Polyclonal Antibody, Unconjugated (bs-11126R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
PVRL1 Polyclonal AntibodyBS-11126R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetNECTIN1
  • SizePrice
Immunohistochemical staining of human skin shows strong membranous positivity in squamous epithelial cells.
Product group Antibodies
Atlas Antibodies
Anti-NECTIN1 AntibodyHPA026846
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetNECTIN1
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Product group Antibodies
LifeSpan BioSciences
Nectin-1 / PVRL1 AntibodyLS-C405545
ApplicationsWestern Blot, ELISA
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TargetNECTIN1
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Nectin 1 antibody detects Nectin 1 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nectin 1 protein stained by Nectin 1 antibody (GTX130824) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Product group Antibodies
References
GeneTex
Nectin 1 antibodyGTX130824
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Rat
TargetNECTIN1
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Product group Antibodies
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