Anti-p21Ras [DWP]

Absolute Antibody

Anti-p21Ras [DWP]

9

This antibody recognizes human mutant H-, K-, and N-Ras proteins. This antibody may also react with other Ras mutants with a hydrophobic amino acid (V, A, C, H, I, L, M, Y, T, N, Q) at position 12 (PMID: 9440050). This ras protein binds GDP/GTP and posses

This antibody was capable of binding G12V and G12C mutant peptides of human ras protein in competition ELISAs (PMID: 3094010). It was reported to cross react with peptides containing amino acids V, A, C, H, I, L, M, Y, T, N, Q at position 12 in an ELISA (PMID: 9440050). This antibody could detect mutant KRAS proteins from cell lysates of human tumor cell lines in a western blot, but did not react with normal KRAS or other KRAS mutants. Immunohistochemical studies showed that DWP-labeled transformed NIH cells and human carcinoma cells contained p21ras is with either Val or Cys at position 12 but not normal or other activated KRAS protein (PMID: 3094010).

Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

DWP

HRAS

HRas proto-oncogene, GTPase

C-BAS/HAS; C-HA-RAS1; c-has/bas p21 protein; C-H-RAS; CTLO; GTP- and GDP-binding peptide B; GTPase HRas; HAMSV; Ha-Ras1 proto-oncoprotein; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; HRAS1; H-RASIDX; p19 H-RasIDX protein; p21ras; Ras family small GTP binding protein H-Ras; RASH1; transformation gene: oncogene HAMSV; transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Rabbit

IgG

GTPase NRas

Human

-20°C,2°C to 8°C

12352203