Anti-p21Ras [DWP]

Absolute Antibody

Anti-p21Ras [DWP]

9

This antibody was capable of binding G12V and G12C mutant peptides of human ras protein in competition ELISAs (PMID: 3094010). It was reported to cross react with peptides containing amino acids V, A, C, H, I, L, M, Y, T, N, Q at position 12 in an ELISA (PMID: 9440050). This antibody could detect mutant KRAS proteins from cell lysates of human tumor cell lines in a western blot, but did not react with normal KRAS or other KRAS mutants. Immunohistochemical studies showed that DWP-labeled transformed NIH cells and human carcinoma cells contained p21ras is with either Val or Cys at position 12 but not normal or other activated KRAS protein (PMID: 3094010).

Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

DWP

HRAS

HRas proto-oncogene, GTPase

C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras, GTPase HRas, GTP- and GDP-binding peptide B, Ha-Ras1 proto-oncoprotein, Harvey rat sarcoma viral oncogene homolog, Harvey rat sarcoma viral oncoprotein, Ras family small GTP binding protein H-Ras, c-has/bas p21 protein, p19 H-RasIDX protein, transformation gene: oncogene HAMSV, transforming protein p21, v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Mouse

IgG2b

GTPase NRas

-20°C,2°C to 8°C

12352203