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Western Blot analysis of 293 cells using p27 Polyclonal Antibody
Western Blot analysis of 293 cells using p27 Polyclonal Antibody
Western Blot analysis of 293 cells using p27 Polyclonal Antibody

CDKN1B Antibody

CSB-PA003625
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDKN1B
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDKN1B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1027
  • Target name
    CDKN1B
  • Target description
    cyclin dependent kinase inhibitor 1B
  • Target synonyms
    CDKN4; cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); KIP1; MEN1B; MEN4; P27KIP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46527
  • Protein Name
    Cyclin-dependent kinase inhibitor 1B
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 3. Flow Cytometry analysis of SiHa cells using anti-p27 KIP 1/CDKN1B antibody (PB9070). Overlay histogram showing SiHa cells stained with PB9070 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p27 KIP 1/CDKN1B Antibody (PB9070, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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