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Western Blot analysis of Jurkat cells using PAI-1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using PAI-1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using PAI-1 Polyclonal Antibody

SERPINE1 Antibody

CSB-PA003699
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSERPINE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SERPINE1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5054
  • Target name
    SERPINE1
  • Target description
    serpin family E member 1
  • Target synonyms
    PAI, PAI-1, PAI1, PLANH1, plasminogen activator inhibitor 1, endothelial plasminogen activator inhibitor, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, serpin E1, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05121
  • Protein Name
    Plasminogen activator inhibitor 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PAI1 using anti-PAI1 antibody (A00637-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAI1 antigen affinity purified polyclonal antibody (Catalog # A00637-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAI1 at approximately 45-50KD. The expected band size for PAI1 is at 45KD.
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