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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA017492DSR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA017492DSR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA017492DSR1HU at dilution of 1:100

PAX6 Antibody

CSB-PA017492DSR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPAX6
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAX6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5080
  • Target name
    PAX6
  • Target description
    paired box 6
  • Target synonyms
    AN; AN1; AN2; Aniridia 1; Aniridia 2; aniridia type II protein; ASGD5; D11S812E; FVH1; MGDA; oculorhombin; paired box homeotic gene-6; paired box protein Pax-6; WAGR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26367
  • Protein Name
    Paired box protein Pax-6
  • Scientific Description
    Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAX6 using anti-PAX6 antibody (PB9768). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX6 antigen affinity purified polyclonal antibody (Catalog # PB9768) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX6 at approximately 47 kDa. The expected band size for PAX6 is at 47 kDa.
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