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Immunohistochemical staining of human renal cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemical staining of human renal cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemical staining of human renal cancer shows moderate to strong nuclear positivity in tumor cells.

Anti-PAX8 Antibody

AMAB91488
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPAX8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PAX8 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL7834
  • Conjugate
    Unconjugated
  • Gene ID7849
  • Target name
    PAX8
  • Target description
    paired box 8
  • Target synonyms
    PAX-8, paired box protein Pax-8, paired domain gene 8
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ06710
  • Protein Name
    Paired box protein Pax-8
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PAX8 using anti-PAX8 antibody (A00943-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW579 cell lysate, Lane 2: human SW579 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX8 antigen affinity purified polyclonal antibody (Catalog # A00943-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX8 at approximately 48KD. The expected band size for PAX8 is at 48KD.
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