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ProductsBack/Goat anti-PAX8 (N Terminus)

Goat anti-PAX8 (N Terminus)

EB05513
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPAX8
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PAX8 (N Terminus) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7849
  • Target name
    PAX8
  • Target description
    paired box 8
  • Target synonyms
    paired box protein Pax-8; paired domain gene 8
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1. GeneIDs all Nonhuman: 18510 (mouse); 81819 (rat);. Purification: Antigen affinity purified. Names and symbols: PAX8; paired box gene 8; paired domain gene 8; paired box 8
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAX8 using anti-PAX8 antibody (A00943-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW579 cell lysate, Lane 2: human SW579 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX8 antigen affinity purified polyclonal antibody (Catalog # A00943-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX8 at approximately 48KD. The expected band size for PAX8 is at 48KD.
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