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Western Blot analysis of K562 cells using Pax-8 Polyclonal Antibody
Western Blot analysis of K562 cells using Pax-8 Polyclonal Antibody
Western Blot analysis of K562 cells using Pax-8 Polyclonal Antibody

PAX8 Antibody

CSB-PA003712
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPAX8
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAX8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7849
  • Target name
    PAX8
  • Target description
    paired box 8
  • Target synonyms
    PAX-8, paired box protein Pax-8, paired domain gene 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06710
  • Protein Name
    Paired box protein Pax-8
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAX8 using anti-PAX8 antibody (A00943-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW579 cell lysate, Lane 2: human SW579 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX8 antigen affinity purified polyclonal antibody (Catalog # A00943-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX8 at approximately 48KD. The expected band size for PAX8 is at 48KD.
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