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Immunohistochemistry analysis in human kidney and tonsil tissues using Anti-PBLD antibody. Corresponding PBLD RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human kidney and tonsil tissues using Anti-PBLD antibody. Corresponding PBLD RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human kidney and tonsil tissues using Anti-PBLD antibody. Corresponding PBLD RNA-seq data are presented for the same tissues.

Anti-PBLD Antibody

HPA038035
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPBLD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PBLD Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64081
  • Target name
    PBLD
  • Target description
    phenazine biosynthesis like protein domain containing
  • Target synonyms
    HEL-S-306, MAWBP, MAWDBP, phenazine biosynthesis-like domain-containing protein, MAWD-binding protein, epididymis secretory protein Li 306, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30039
  • Protein Name
    Phenazine biosynthesis-like domain-containing protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PBLD using anti-PBLD antibody (A10149-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBLD antigen affinity purified polyclonal antibody (Catalog # A10149-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PBLD at approximately 32 kDa. The expected band size for PBLD is at 32 kDa.
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Western blot All lanes: PBLD antibody at 6microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa
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Immunohistochemistry analysis in human kidney and tonsil tissues using Anti-PBLD antibody. Corresponding PBLD RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
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Immunohistochemistry analysis in human kidney and tonsil tissues using Anti-PBLD antibody. Corresponding PBLD RNA-seq data are presented for the same tissues.
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TargetPBLD
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