Anti-PD-L1 [10F.9G2]

Absolute Antibody

Anti-PD-L1 [10F.9G2]

9

This antibody is specific for murine PD-L1.

The specificity of this antibody has been confirmed in ELISA analysis (Eppihimer et al, 2002). This antibody reacts specifically with mPD-L1-transfected cells in flow cytometry, and has been used to assess microvascular endothelial cells PD-L1 expression (Eppihimer et al, 2002). When administered to mice, a radiolabelled version of this antibody has been used to quantify PD-L1 expression in vivo, by measuring antibody accumulation in different tissues (Eppihimer et al, 2002). This antibody has also been used in immunohistochemical analysis of PD-L1 expression in frozen tissue sections of murine brain (Eppihimer et al, 2002) and murine hearts (Rodig et al, 2003). This antibody has been shown to block the binding of PD-L1 to both PD-1 and B7-1 (Paterson et al, 2011). This antibody precipitates diabetes in NOD mice and in adoptive transfer models of CD4+ and CD8+ T cell-driven diabetes, and accelerates diabetes in recipients of T cells from diabetic and prediabetic mice (Paterson et al, 2011).

Flow Cytometry, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking

Research Use Only

Monoclonal

10F.9G2

Cd274

CD274 antigen

A530045L16Rik; B7 homolog 1; B7h1; PD-; PDCD1 ligand 1; Pdcd1l; Pdcd1l1; Pdcd1lg1; Pdl1; programmed cell death 1 ligand 1

Rabbit

IgG

Programmed cell death 1 ligand 1

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203