Bio-Connect

Anti-PD-L1 [mAb #18 (PD-L1.A)]

Ab03505-1.1
Absolute Antibody
ApplicationsWestern Blot, Neutralisation/Blocking, Other Application
Product group Antibodies
TargetCD274
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-PD-L1 [mAb #18 (PD-L1.A)]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The antibody could significantly inhibit the binding of PD-1 and PD-L1 at the cellular level. The humanized version of the antibody was constructed. The humanized version of the antibody detected the PD-L1 by western blot analysis. The binding affinity of the mouse and humanized version of the antibody to PD-L1 was measured by surface plasmon resonance, the affinity was respectively 8.81 nM 7.77 nM. In vivo studies demonstrated that the humanized version of the antibody could effectively inhibit tumour growth showing potential tumour therapeutic value (CN109232740). This antibody was also used in the generation of a bispecific antibody (BsAb) in a dual-variable-domain immunoglobulin (DVD-Ig) format targeting CD47 and PD-L1. The variable-domain from the PD-L1-blocking mAb #18 was introduced in tandem onto h4C1 to generate a dual variable domain bsAb that binds bivalently to both CD47 and PD-L1. This BsAb retained blocking activity for both interactions, but lacked the hemagglutinating activity of the parental m4C1 (Shi et al.; 2020; PMID: 32296041; Kaur et al.; 2020; PMID: 33244513).
  • Applications
    Western Blot, Neutralisation/Blocking, Other Application
  • Applications Supplier
    Inhibition; SPR; WB; in vivo
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    mAb #18 (PD-L1.A)
  • Gene ID29126
  • Target name
    CD274
  • Target description
    CD274 molecule
  • Target synonyms
    B7 homolog 1; B7-H; B7H1; CD274 antigen; hPD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; programmed cell death 1 ligand 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9NZQ7
  • Protein Name
    Programmed cell death 1 ligand 1
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original format for improved compatibility with existing reagents assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing BALB/c mice with PD-L1.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Anti-PD-L1 Antibody (Biotin)
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCD274
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsFlow Cytometry
TargetCD274
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCD274
  • SizePrice
Product group Antibodies
Assay Genie
ApplicationsFlow Cytometry, ImmunoHistoChemistry
TargetCD274
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsELISA
TargetCD274
  • SizePrice
FluoSite(tm) Anti-PD-L1 Antibody, PE-labeled
Product group Antibodies
BPS Bioscience
ApplicationsImmunoFluorescence
TargetCD274
  • SizePrice
Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA878942ESR1HUat dilution of 1:100
Product group Antibodies
Cusabio
CD274 AntibodyCSB-PA878942ESR1HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCD274
  • SizePrice
Product group Antibodies
MedChem Express
TargetCD274
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry
TargetCD274
  • SizePrice