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ProductsBack/Goat anti-CD274 / PD-L1, Biotinylated

Goat anti-CD274 / PD-L1, Biotinylated

EB06621-B
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCD274
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CD274 / PD-L1, Biotinylated Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Gene ID29126
  • Target name
    CD274
  • Target description
    CD274 molecule
  • Target synonyms
    ADMIO5, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1, programmed cell death 1 ligand 1, B7 homolog 1, CD274 antigen, PDCD1 ligand 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_054862.1; NP_001254635.1. Purification: Antigen affinity purified. Names and symbols: CD274; CD274 molecule; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PD-L1/B7-H1 using anti-PD-L1/B7-H1 antibody (A00109). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1/B7-H1 antigen affinity purified polyclonal antibody (Catalog # A00109) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1/B7-H1 at approximately 62KD. The expected band size for PD-L1/B7-H1 is at 33KD.
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