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Immunohistochemical staining of human tonsil shows strong immunoreactivity in a subset of lymphoid cells, mainly in the reaction centrum.
Immunohistochemical staining of human tonsil shows strong immunoreactivity in a subset of lymphoid cells, mainly in the reaction centrum.
Immunohistochemical staining of human tonsil shows strong immunoreactivity in a subset of lymphoid cells, mainly in the reaction centrum.

Anti-PDCD1 Antibody

AMAB91197
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPDCD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDCD1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL3624
  • Conjugate
    Unconjugated
  • Gene ID5133
  • Target name
    PDCD1
  • Target description
    programmed cell death 1
  • Target synonyms
    CD279; hPD-1; hPD-l; hSLE1; PD1; PD-1; programmed cell death 1 protein; programmed cell death protein 1; protein PD-1; SLEB2; systemic lupus erythematosus susceptibility 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15116
  • Protein Name
    Programmed cell death protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PD1/PDCD1 using anti-PD1/PDCD1 antibody (RP1039). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD1/PDCD1 antigen affinity purified polyclonal antibody (Catalog # RP1039) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD1/PDCD1 at approximately 65KD. The expected band size for PD1/PDCD1 is at 32KD.
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