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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PDGFR alpha Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PDGFR alpha Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PDGFR alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PDGFR alpha Antibody

A30446
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDGFRA
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PDGFR alpha Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Gene ID5156
  • Target name
    PDGFRA
  • Target description
    platelet derived growth factor receptor alpha
  • Target synonyms
    CD140A, PDGFR-2, PDGFR2, platelet-derived growth factor receptor alpha, CD140 antigen-like family member A, CD140a antigen, PDGF-R-alpha, alpha-type platelet-derived growth factor receptor, platelet-derived growth factor receptor 2, platelet-derived growth factor receptor, alpha polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16234
  • Protein Name
    Platelet-derived growth factor receptor alpha
  • Scientific Description
    Boster Bio Anti-PDGFR alpha Antibody (Catalog# A30446). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zhu Z, Ma Q, Meng X, et al. Interstitial Cell Dysregulation in Allergic Contact Dermatitis: A Morphodynamic Study of Novel Interstitial Cell Telocytes. Microsc Microanal. 2023,29(2):762-776. doi: 10.1093/micmic/ozad010
    Read this paper

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Figure 1. Western blot analysis of PDGFRA using anti-PDGFRA antibody (A00366). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRA antigen affinity purified polyclonal antibody (Catalog # A00366) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGFRA at approximately 180 kDa. The expected band size for PDGFRA is at 123,180 kDa.
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