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Anti-PDIA6 Antibody

A31840
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-PDIA6 Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    >95%
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to PDIA6
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDIA6 using anti-PDIA6 antibody (A03813-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat lung tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIA6 antigen affinity purified polyclonal antibody (Catalog # A03813-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDIA6 at approximately 50KD. The expected band size for PDIA6 is at 50KD.
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