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PDIA6 antibody

70R-5434
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    PDIA6 antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Western blot All lanes: PDIA6 antibody at 5.9microg/ml Lane 1: Mouse heart tissue Lane 2: Mouse lung tissue Lane 3: Mouse liver tissue Lane 4: 293T whole cell lysate Lane 5: Hela whole cell lysate Lane 6: HL60 whole cell lysate Lane 7: HepG2 whole cell lysate Lane 8: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 54, 48 kDa Observed band size: 54 kDa
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Figure 1. Western blot analysis of PDIA6 using anti-PDIA6 antibody (A03813-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat lung tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIA6 antigen affinity purified polyclonal antibody (Catalog # A03813-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDIA6 at approximately 50KD. The expected band size for PDIA6 is at 50KD.
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Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using HPA034652 antibody. Corresponding PDIA6 RNA-seq data are presented for the same tissues.
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