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ProductsBack/PDIA6 antibody [N1N3]

PDIA6 antibody [N1N3]

GTX121275
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPDIA6
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Overview

  • Supplier
    GeneTex
  • Product Name
    PDIA6 antibody [N1N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10130
  • Target name
    PDIA6
  • Target description
    protein disulfide isomerase family A member 6
  • Target synonyms
    endoplasmic reticulum protein 5; epididymis secretory sperm binding protein; ER protein 5; ERP5; P5; protein disulfide isomerase P5; protein disulfide isomerase-associated 6; protein disulfide isomerase-related protein; protein disulfide-isomerase A6; thioredoxin domain containing 7 (protein disulfide isomerase); thioredoxin domain-containing protein 7; TXNDC7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15084
  • Protein Name
    Protein disulfide-isomerase A6
  • Scientific Description
    Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (Hayano and Kikuchi, 1995 [PubMed 7590364]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • IRE1-XBP1 pathway regulates oxidative proinsulin folding in pancreatic beta cells. Tsuchiya Y et al., 2018 Apr 2, J Cell Biol
    Read more

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Figure 1. Western blot analysis of PDIA6 using anti-PDIA6 antibody (A03813-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat lung tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIA6 antigen affinity purified polyclonal antibody (Catalog # A03813-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDIA6 at approximately 50KD. The expected band size for PDIA6 is at 50KD.
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